iPSC-derived Neurons

Study of iPSC-derived Neurons 

MaxOne and MaxTwo are best suited for long-term and label-free analysis of induced pluripotent stem cell (iPSC) -derived neurons.

  • Mono-culture and co-culture (e.g., with astrocytes)
  • Disease model cells
  • Spheroids and Organoids

Webinar

Watch our iPSC webinar that introduces high-resolution functional imaging of human iPSC-derived neurons

What You Can Do

Evaluate Cell Function During Development
Track Cell Culture Activity Across Multiple Days and Obtain high-resolution activity maps of iPSC cultures using electrical activity

*Data from iPSC-derived Dopaminergic Neurons

 

MaxOne and MaxTwo enable recording of neuronal activity and label-free electrical imaging of whole samples. The extracellular action potential (EAP) activity map obtained provides information on:

  • Location of spontaneously active cells
  • EAP amplitude
  • Spike rate

Characterize Network Connectivity and Synchrony
Analyze functional connectivity and synchrony of iPSC-derived neuronal cultures

MaxOne and MaxTwo allow long-term tracking of active neurons. Connectivity and synchrony can be extracted from:

  • Network burst features (burst frequency, length, amplitude, etc.)
  • Pairwise correlations in neuronal spike trains
  • Response to selective electrical stimulation

Investigate Cell Maturity and Excitability
Study propagation of action potentials along axonal arbors of hundreds of iPSC-derived neurons simultaneously

MaxOne and MaxTwo capture the activity of neurons at high signal quality and unprecedented spatio-temporal resolution, allowing the detection of action potentials along axons.

  • Observe axonal action potential propagation of single neurons across multiple days.
  • Analyze axonal conduction velocity.
  • Compare axonal action potential propagation between healthy and disease model cell lines.
Download Application Brochure

Selected Publications

A Multimodal Fitting Approach to Construct Single-Neuron Models with Patch Clamp and High-Density Microelectrode Arrays

Buccino, Alessio Paolo; Damart, Tanguy; Bartram, Julian; Mandge, Darshan; Xue, Xiaohan; Zbili, Mickael; Gänswein, Tobias; Jaquier, Aurélien; Emmenegger, Vishalini; Markram, Henry; Hierlemann, Andreas; Geit, Werner Van

A Multimodal Fitting Approach to Construct Single-Neuron Models with Patch Clamp and High-Density Microelectrode Arrays Journal Article

Neural Computation (MIT Press), 2024.

Abstract | Links | BibTeX

An implantable biohybrid nerve model towards synaptic deep brain stimulation

Sifringer, Léo; Fratzl, Alex; Clément, Blandine F; Chansoria, Parth; Mönkemöller, Leah S; Duru, Jens; Ihle, Stephan J; Steffens, Simon; Beltraminelli, Anna; Ceylan, Eylul; Hengsteler, Julian; Maurer, Benedikt; Weaver, Sean M; Tringides, Christina M; Vulić, Katarina; Madduri, Srinivas; Zenobi-Wong, Marcy; Roska, Botond; Vörös, János; Ruff, Tobias

An implantable biohybrid nerve model towards synaptic deep brain stimulation Journal Article

bioRxiv, 2024.

Abstract | Links | BibTeX

Biological Neurons Compete with Deep Reinforcement Learning in Sample Efficiency in a Simulated Gameworld

Khajehnejad, Moein; Habibollahi, Forough; Paul, Aswin; Razi, Adeel; Kagan, Brett J

Biological Neurons Compete with Deep Reinforcement Learning in Sample Efficiency in a Simulated Gameworld Journal Article

arXiv , 2024.

Abstract | Links | BibTeX

Analgesic Effect of Botulinum Toxin in Neuropathic Pain is Sodium Channel Independent

Kesdoğan, Aylin B; Neureiter, Anika; Gaebler, Arnim J; Kalia, Anil K; Körner, Jannis; Lampert, Angelika

Analgesic Effect of Botulinum Toxin in Neuropathic Pain is Sodium Channel Independent Journal Article

Neuropharmacology, 2024.

Abstract | Links | BibTeX

Forskolin-driven conversion of human somatic cells into induced neurons through regulation of the cAMP-CREB1-JNK signaling

Wang, Guodong; Zhang, Dandan; Qin, Liangshan; Liu, Quanhui; Tang, Wenkui; Liu, Mingxing; Xu, Fan; Tang, Fen; Cheng, Leping; Mo, Huiming; Yuan, Xiang; Wang, Zhiqiang; Huang, Ben

Forskolin-driven conversion of human somatic cells into induced neurons through regulation of the cAMP-CREB1-JNK signaling Journal Article

Theranostics , 2024.

Abstract | Links | BibTeX

DeePhys: A machine learning–assisted platform for electrophysiological phenotyping of human neuronal networks

Hornauer, Philipp; Prack, Gustavo; Anastasi, Nadia; Ronchi, Silvia; Kim, Taehoon; Donner, Christian; Fiscella, Michele; Borgwardt, Karsten; Taylor, Verdon; Jagasia, Ravi; Roqueiro, Damian; Hierlemann, Andreas; Schröter, Manuel

DeePhys: A machine learning–assisted platform for electrophysiological phenotyping of human neuronal networks Journal Article

Stem Cell Reports, 2024.

Abstract | Links | BibTeX

Active Inference and Intentional Behaviour

Friston, Karl J; Salvatori, Tommaso; Isomura, Takuya; Tschantz, Alexander; Kiefer, Alex; Verbelen, Tim; Koudahl, Magnus; Paul, Aswin; Parr, Thomas; Razi, Adeel; Kagan, Brett; Buckley, Christopher L; and Ramstead, Maxwell J D

Active Inference and Intentional Behaviour Journal Article

arXiv, 2023.

Abstract | Links | BibTeX

Hyperexcitability and translational phenotypes in a preclinical model of SYNGAP1mutations

Silverman, Jill L; Fenton, Timothy; Haouchine, Olivia; Hallam, Elizabeth; Smith, Emily; Jackson, Kiya; Rahbarian, Darlene; Canales, Cesar; Adhikari, Anna; Nord, Alex; Ben-Shalom, Roy

Hyperexcitability and translational phenotypes in a preclinical model of SYNGAP1mutations Journal Article

Research Square, 2023.

Abstract | Links | BibTeX

Critical dynamics arise during structured information presentation within embodied in vitro neuronal networks

Habibollahi, Forough; Kagan, Brett J; Burkitt, Anthony N; French, Chris

Critical dynamics arise during structured information presentation within embodied in vitro neuronal networks Journal Article

Nature Communications, 2023.

Abstract | Links | BibTeX

Functional imaging of conduction dynamics in cortical and spinal axons

Radivojevic, Milos; Punga, Anna Rostedt

Functional imaging of conduction dynamics in cortical and spinal axons Journal Article

eLife, 2023.

Abstract | Links | BibTeX

Investigation of the input-output relationship of engineered neural networks using high-density microelectrode arrays

Duru, Jens; Maurer, Benedikt; Doran, Ciara Giles; Jelitto, Robert; Küchler, Joël; Ihle, Stephan J; Ruff, Tobias; John, Robert; Genocchi, Barbara; Vörös, János

Investigation of the input-output relationship of engineered neural networks using high-density microelectrode arrays Journal Article

Biosensors and Bioelectronics, 2023.

Abstract | Links | BibTeX

Development of a Hypersensitivity Evaluation Method for Cultured Sensory Neurons Using Electrical Activity Recording

Miyahara, Yuki; Shimba, Kenta; Kotani, Kiyoshi; Jimbo, Yasuhiko

Development of a Hypersensitivity Evaluation Method for Cultured Sensory Neurons Using Electrical Activity Recording Conference

IEEE EMBC 2023 2023.

Abstract | Links | BibTeX

Neural Activity and Information Processing Capacity of Neuronal Culture

Akita, Dai; Suwa, Eisuke; Ikeda, Narumitsu; Takahashi, Hirokazu

Neural Activity and Information Processing Capacity of Neuronal Culture Conference

IEEE EMBC 2023 2023.

Abstract | Links | BibTeX

Microfluidic technologies for reconstituting neuronal network functions in vitro

Yamamoto, Hideaki; Hirano-Iwata, Ayumi; Sato, Shigeo

Microfluidic technologies for reconstituting neuronal network functions in vitro Journal Article

JSAP Review, 2023.

Abstract | Links | BibTeX

Measuring Neuronal Network Activity Using Human Induced Neuronal Cells - Stem Cell-Based Neural Model Systems for Brain Disorders

McSweeney, Danny; English, Jay; Howell, Ethan; Ribbe, Fumiko; Pak, ChangHui

Measuring Neuronal Network Activity Using Human Induced Neuronal Cells - Stem Cell-Based Neural Model Systems for Brain Disorders Book Chapter

Methods in Molecular Biology, 2023.

Abstract | Links | BibTeX

Cultured Vagal Afferent Neurons as Sensors for Intestinal Effector Molecules

Girardi, Gregory; Zumpano, Danielle; Goshi, Noah; Raybould, Helen; Seker, Erkin

Cultured Vagal Afferent Neurons as Sensors for Intestinal Effector Molecules Journal Article

biosensors, 2023.

Abstract | Links | BibTeX

The potential of in vitro neuronal networks cultured on Micro Electrode Arrays for biomedical research

Cerina, Marta; Piastra, Maria Carla; Frega, Monica

The potential of in vitro neuronal networks cultured on Micro Electrode Arrays for biomedical research Journal Article

Progress in Biomedical Engineering, 2023.

Abstract | Links | BibTeX

Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells

Xu, He Jax; Yao, Yao; Yao, Fenyong; Chen, Jiehui; Li, Meishi; Yang, Xianfa; Li, Sheng; Lu, Fangru; Hu, Ping; He, Shuijin; Peng, Guangdun; Jing, Naihe

Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells Journal Article

Cell Regeneration, 2023.

Abstract | Links | BibTeX

Multiplex epigenome editing of MECP2 to rescue Rett syndrome neurons

Qian, Junming; Guan, Xiaonan; Xie, Bing; Xu, Chuanyun; Niu, Jacqueline; Tang, Xin; Li, Charles H; Colecraft, Henry M; Jaenisch, Rudolf; Liu, Shawn X

Multiplex epigenome editing of MECP2 to rescue Rett syndrome neurons Journal Article

Science Translational Medicine, 2023.

Abstract | Links | BibTeX

CASK loss of function differentially regulates neuronal maturation and synaptic function in human induced cortical excitatory neurons

McSweeney, Danny; Gabriel, Rafael; Jin, Kang; Pang, Zhiping P; Aronow, Bruce; and Pak, ChangHui

CASK loss of function differentially regulates neuronal maturation and synaptic function in human induced cortical excitatory neurons Journal Article

iScience, 2022.

Abstract | Links | BibTeX

In vitro neurons learn and exhibit sentience when embodied in a simulated game-world

Kagan, Brett J; Kitchen, Andy C; Tran, Nhi T; Habibollahi, Forough; Khajehnejad, Moein; Parker, Bradyn J; Bhat, Anjali; Rollo, Ben; Razi, Adeel; Friston, Karl J

In vitro neurons learn and exhibit sentience when embodied in a simulated game-world Journal Article

Neuron, 2022.

Abstract | Links | BibTeX

Long-term morphological and functional dynamics of human stem cell-derived neuronal networks on high-density micro-electrode arrays

Habibey, Rouhollah; Striebel, Johannes; Schmieder, Felix; Czarske, Jürgen; Busskamp, Volker

Long-term morphological and functional dynamics of human stem cell-derived neuronal networks on high-density micro-electrode arrays Journal Article

Frontiers in Neuroscience, 2022.

Abstract | Links | BibTeX

A multi-modal fitting approach to construct single-neuron models with patch clamp and high-density microelectrode arrays

Buccino Alessio Paolo; Damart, Tanguy; Bartram Julian; Mandge Darshan; Xue Xiaohan; Zbili Mickael; Gänswein Tobias; Jaquier Aurélien; Emmenegger Vishalini; Markram Henry; Hierlemann Andreas; Van Geit Werner.

A multi-modal fitting approach to construct single-neuron models with patch clamp and high-density microelectrode arrays Journal Article

bioRxiv, 2022.

Abstract | Links | BibTeX

Inferring monosynaptic connections from paired dendritic spine Ca2+ imaging and large-scale recording of extracellular spiking

Xue, Xiaohan; Buccino, Alessio Paolo; Kumar, Sreedhar Saseendran; Hierlemann, Andreas; Bartram, Julian

Inferring monosynaptic connections from paired dendritic spine Ca2+ imaging and large-scale recording of extracellular spiking Journal Article

Journal of Neural Engineering, 2022.

Abstract | Links | BibTeX

Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells

Xu, Jax H; Yao, Yao; Yao, Fenyong; Chen, Jiehui; Li, Meishi; Xianfa, ; Yang, ; Li, Sheng; Lu, Fangru; Hu, Ping; He, Shuijin; Peng, Guangdun; Jing, Naihe

Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells Journal Article

BioRxiv, 2022.

Abstract | Links | BibTeX

In vitro neurons learn and exhibit sentience when embodied in a simulated game-world

Kagan, Brett J; Kitchen, Andy C; Tran, Nhi T; Parker, Bradyn J; Bhat, Anjali; Rollo, Ben; Razi, Adeel; Friston, Karl J

In vitro neurons learn and exhibit sentience when embodied in a simulated game-world Journal Article

bioRxiv, 2021.

Abstract | Links | BibTeX

Unifying framework for information processing in stochastically driven dynamical systems

Kubota, Tomoyuki; Takahashi, Hirokazu; and Nakajima, Kohei

Unifying framework for information processing in stochastically driven dynamical systems Journal Article

Physical Review Research, 2021.

Abstract | Links | BibTeX

Depth Recording Capabilities of Planar High-Density Microelectrode Arrays

Frey, U; Egert, U; Jäckel, D; Sedivy, J; Ballini, M; Livi, P; Faraci, F; F. Heer, Hafizovic S; Roscic, B; Hierlemann, A

Depth Recording Capabilities of Planar High-Density Microelectrode Arrays Conference

2009 4th International IEEE/EMBS Conference on Neural Engineering 2009.

Abstract | Links | BibTeX

A perforated CMOS microchip platform for immobilization and activity monitoring of electrogenic cells

Greve, Frauke; Lichtenberg, Jan; Kirstein, Kay Uwe; Frey, Urs; Perriard, Jean Claude; Hierlemann, Andreas

A perforated CMOS microchip platform for immobilization and activity monitoring of electrogenic cells Journal Article

Journal of Micromechanics and Microengineering, 17 (3), pp. 462-471, 2007, ISSN: 0960-1317.

Abstract | Links | BibTeX

CMOS microelectrode array for bidirectional interaction with neuronal networks

Heer, Flavio; Hafizovic, Sadik; Franks, Wendy; Blau, Axel; Ziegler, Christiane; Hierlemann, Andreas

CMOS microelectrode array for bidirectional interaction with neuronal networks Journal Article

IEEE Journal of Solid-State Circuits, 41 (7), pp. 1620-1629, 2006, ISSN: 0018-9200.

Abstract | Links | BibTeX

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